Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Hyaluronan Production by Renomedullary Interstitial Cells: Influence of Endothelin, Angiotensin II and Vasopressin.
The Swedish Red Cross University College, Department of Health Sciences. Uppsala University.ORCID iD: 0000-0003-4092-2854
Uppsala University.
Hoshi University, Tokyo, Japan.
Hoshi University, Tokyo, Japan.
Show others and affiliations
2017 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 18, no 12, article id 2701Article in journal (Refereed) Published
Abstract [en]

The content of hyaluronan (HA) in the interstitium of the renal medulla changes in relation to body hydration status. We investigated if hormones of central importance for body fluid homeostasis affect HA production by renomedullary interstitial cells in culture (RMICs). Simultaneous treatment with vasopressin and angiotensin II (Ang II) reduced HA by 69%. No change occurred in the mRNA expressions of hyaluronan synthase 2 (HAS2) or hyaluronidases (Hyals), while Hyal activity in the supernatant increased by 67% and CD44 expression reduced by 42%. The autocoid endothelin (ET-1) at low concentrations (10-10 and 10-8 M) increased HA 3-fold. On the contrary, at a high concentration (10-6 M) ET-1 reduced HA by 47%. The ET-A receptor antagonist BQ123 not only reversed the reducing effect of high ET-1 on HA, but elevated it to the same level as low concentration ET-1, suggesting separate regulating roles for ET-A and ET-B receptors. This was corroborated by the addition of ET-B receptor antagonist BQ788 to low concentration ET-1, which abolished the HA increase. HAS2 and Hyal2 mRNA did not alter, while Hyal1 mRNA was increased at all ET-1 concentrations tested. Hyal activity was elevated the most by high ET-1 concentration, and blockade of ET-A receptors by BQ123 prevented about 30% of this response. The present study demonstrates an important regulatory influence of hormones involved in body fluid balance on HA handling by RMICs, thereby supporting the concept of a dynamic involvement of interstitial HA in renal fluid handling.

Place, publisher, year, edition, pages
2017. Vol. 18, no 12, article id 2701
Keywords [en]
angiotensin II, endothelin, hyaluronan, interstitium, kidney, medulla, vasopressin
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:rkh:diva-2511DOI: 10.3390/ijms18122701PubMedID: 29236055OAI: oai:DiVA.org:rkh-2511DiVA, id: diva2:1166229
Funder
Swedish Research CouncilAvailable from: 2017-12-14 Created: 2017-12-14 Last updated: 2018-01-13Bibliographically approved

Open Access in DiVA

fulltext(4150 kB)3 downloads
File information
File name FULLTEXT01.pdfFile size 4150 kBChecksum SHA-512
ec255650121fbfdcb40f36032c170c80926fde752e7dd70215216828731adc8f0e541aaf80ea0cb3286dcc30e4c21206577cb129405fb989f8a6858e497c5aee
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Stridh, Sara

Search in DiVA

By author/editor
Stridh, Sara
By organisation
Department of Health Sciences
In the same journal
International Journal of Molecular Sciences
Pharmacology and Toxicology

Search outside of DiVA

GoogleGoogle Scholar
Total: 3 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 67 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf